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Conte has been critical of the Italian soccer

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MessagePosté le: Mer 11 Avr - 05:33 (2018)    Sujet du message: Conte has been critical of the Italian soccer Répondre en citant

MILAN , Nov. 18 (Xinhua) -- Italy head coach Antonio Conte warned on Tuesday that Italy soccer is not developing in the right track, and called for actions taken to deal with the problem.

Conte has been critical of the Italian soccer which he reckons should embrace change to improve.

"I know that Italian soccer isn't developing in the right direction," Conte said ahead of the Italy - Albania friendly in Genoa. "We must come to terms with this and then turn words into actions."

He advised that the Italian soccer should make concerted efforts in order to overcome current crisis.

"We need the involvement of all parties concerned. If it continues at this rate then it will be just the beginning of a decline ," he said.

"When I was at Juventus I thought only of my club, but from this position I see things that I do not like," he added.
why temperature control in the IVF lab is crucial?

Temperature control in the IVF lab is crucial , as it plays an important role in the success of any IVF cycle.

The primary function of an ART laboratory is to provide an optimal environment for gametes and embryos to grow. The success of any ART cycle is very much dependant on the Quality Control (QC) and Quality Assurance (QA) maintained in the IVF laboratory. Optimization of the laboratory procedures is of great interest to the scientists in the field of IVF.

All important IVF equipment's used in IVF laboratory has direct or indirect contact with embryos, gametes or the culture media. These all should be handled and monitored in an accurate and precise way. Fine temperature regulation is critical to embryology laboratories aiming to maximize in vitro fertilization (IVF) and development, implantation and pregnancy of assisted reproductive technique (ART).

It is common practice to maintain a temperature of 37 degree C during all working and culture conditions in the IVF laboratory. This will allow for an optimal environment for the handling and culture of gametes and embryos.

The three most important physical conditions in the IVF laboratory that can be controlled are the temperature , pH and osmolality of the IVF culture medium. Laboratories are required to document and monitor these physical conditions regularly as part of their ongoing QCQA programs. Monitoring and documentation of temperatures inside incubators, refrigerators and freezers is an integral part of routine day to day QC in the IVF laboratory.

Temperature measurements and temperature control in the IVF lab are crucial for the success of IVF cycle. All important equipment like the incubators, micro manipulator , refrigerator, freezing machine etc. should be on uninterrupted power supply with trigger alarm feature. There should be a wall mounted data logger displaying the laboratory ambient temperature and humidity levels. All IVF equipment should be on urgent power supply. Crucial equipment like incubators, micro manipulator systems , medical refrigerator, freezing machines etc should be on uninterrupted power supply with a trigger alarm or call back service.

Learn in detail about ivf lab equipment's that are useful to maintain the temperature of 37°C in an ivf lab at http:www.shivaniivffunctionwisefunctionwise.php?val=29#rgt

The optimal temperature range for human IVF culture is assumed to be between the limits of 36.7°C and 37.0°C; deviations above and below this range should be regarded as criticaldangerous. The effects of sub optimal temperature control can be manifested as fragmentation, delayed cell division , or reduced pregnancy rates due to induced chromosomal abnormalities that jeopardize implantation or result in early pregnancy loss, particularly at the pre-clinical stage (biochemical pregnanciesblighted ova). Therefore every effort should be made to ensure that there is no temperature fluctuation during any laboratory manipulation that involves the handling of oocytes or embryos and that the temperature is maintained at 37°C.

Oocytes need to be kept at body temperature (36–37°C) as much as possible. There should also be appropriate equipment in use to maintain this temperature when laboratory and egg collection areas are on different sites. Scanning Petri dishes, collection tubes and heating blocks are pre-warmed at 37°C. Follicular aspirates are checked for the presence of oocyte-cumulus complexes under a stereo dissecting microscope with transmitted illumination base and heated stage. Exposure of oocytes to light should be minimized where possible.

Incubator temperature is one of the most precious variables in IVF labs. When the IVF started , almost all incubator temperatures were set at 37 degrees which mimic in vivo conditions. The majority of embryologists consider culture at 37 degrees is the back bone for the incubators. Embryology laboratories cover almost all IVFICSI-ET treatment needs with at least 2–3 incubators that should be checked daily for proper maintenance of temperature regulation. The absolute need for daily quality control of incubator temperature is justified by

1. The difficulty to maintain a stable temperature because of frequent opening of the incubator door
2. Overnight power failure where incubators indicate the set temperature and the external thermometer indicates a drop of more than 1 °C with deleterious effect on embryo cleavage and development and
3. Degrees of differences at different locations within the same incubator.

Prolonged exposure of cultures to temperatures other than optimal 37 °C, reduces the ability of fertilization and nullifies the ability of cell division or cleavage, growth , implantation potential and subsequent achievement of pregnancy.

The temperature has to be maintained in dish used for embryos and other sample.
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